Circadian rhythm entrainment of the jewel wasp, Nasonia vitripennis, by antagonistic interactions of multiple spectral inputs

Circadian light entrainment in some insects is regulated by blue-light sensitive cryptochrome (CRY) protein that is expressed in the clock neurons, but this is not the case in hymenopterans. The hymenopteran clock does contain CRY, but it appears to be light-insensitive. Therefore, we investigated the role of retinal photoreceptors in the photic entrainment of the jewel wasp Nasonia vitripennis. Application of monochromatic light stimuli at different light intensities caused phase shifts in the wasp’s circadian activity from which an action spectrum with three distinct peaks was derived. Electrophysiological recordings from the compound eyes and ocelli revealed the presence of three photoreceptor classes, with peak sensitivities at 340 nm (ultraviolet), 450 nm (blue), and 530 nm (green). An additional photoreceptor class in the ocelli with sensitivity maximum at 560–580 nm (red) was found. Whereas a simple sum of photoreceptor spectral sensitivities could not explain the action spectrum..., This dataset consists of all processed data (behavioural data and electrophysiology data) needed to reproduce the analysis of the circadian action spectrum in Nasonia vitripennis, as described in the manuscript under the same title. Data included here: the behaviour phase shifts data under different photoperiods and for phase response curve under 14:10 LD, the circadian action spectrum data, and the electrophysiology measurements in the compound eyes and ocelli of Nasonia. All the R/MatLab scripts needed to process the data and reproduce the analysis are also included in this dataset. Briefly: For all phase shift behaviour experiments, Nasonia vitripennis was entrained under a certain light-dark cycle for 7 days. On day 8 some Nasonia was subjected to different light stimulation, and they then were left in the darkness for another 10 days. In total, we tested Nasonia's phase shift responses under 14 wavelengths and 4 different light intensities per wavelength. By constructing this circa..., File extensions of the deposited data files include .xlsx and .csv for the processed data and .R and .m for the scripts.部分昆虫的昼夜光节律同步由表达于时钟神经元中的蓝光敏感隐花色素（cryptochrome, CRY）蛋白调控，但膜翅目昆虫并非如此。尽管膜翅目昆虫的生物钟系统中同样存在CRY蛋白，但其似乎不具备光敏感性。为此，本研究针对丽蝇蛹集金小蜂（Nasonia vitripennis）的昼夜光诱导同步过程中视网膜光感受器的作用展开了探究。施加不同光强的单色光刺激可引发该蜂昼夜活动节律的相位漂移，据此可推导得到具有三个显著峰值的动作光谱。对其复眼与单眼的电生理记录显示，体内存在三类光感受器，峰值灵敏度分别位于340 nm（紫外光）、450 nm（蓝光）与530 nm（绿光）；此外在单眼中还发现了另一类光感受器，其灵敏度峰值位于560~580 nm（红光）。单纯对各类光感受器的光谱灵敏度进行求和，无法解释该动作光谱…… 本数据集包含复现本文同标题研究中丽蝇蛹集金小蜂昼夜光同步动作光谱分析所需的全部处理后数据，涵盖行为学数据与电生理数据。本数据集收录内容如下：不同光周期下的行为相位漂移数据、14:10 LD光暗周期下的相位响应曲线数据、昼夜光同步动作光谱数据，以及丽蝇蛹集金小蜂复眼与单眼的电生理测量数据。本数据集同时附带了用于处理数据、复现分析流程所需的全部R语言与MatLab脚本。 简要说明：所有行为相位漂移实验中，丽蝇蛹集金小蜂均在特定光暗周期下驯化7天。第8天时，部分个体接受不同的光刺激，随后被置于黑暗环境中持续10天。本研究共测试了14种波长下，每种波长对应4种不同光强的金小蜂相位漂移响应。通过构建该昼夜…… 上传数据文件的格式包括：处理后数据采用.xlsx与.csv格式，脚本文件采用.R与.m格式。