Identification of site-specific incorporation of Kacyl/K*acyl in full-length histones in E. coli and mammalian cells

With the evolution of the PylRS system, we identified the PylRS variants for incorporation of all synthesized Kacyl and Kacyl* in the full-length H3 protein both in E. coli and mammalian cells, which covered the tail domain (eg. K23, K27, K56) as well as the globular domain that are difficult for chemical synthesis (eg. K64, K79, K122).