Supplemental Material for Arlt et al., 2020

Supplemental Table 1 contains RT-PCR primer sequences. Supplemental
Table 2 contains primer pairs used in RT-PCR assays. Supplemental Table 3
contains oligos used to generate and genotype transgenic mice. Supplemental
Figure 1 displays a self-symmetry triangular dot plot of the Srsx-containing
ampliconic region on the X. Supplemental
Figure 2 is a dot plot of DNA sequence identity between two Srsx-containing
X amplicons. Supplemental Figure 3
shows low level of expression from the Y of sequences with homology to Laidx. Supplemental Figure 4 shows Laidx expression exclusively in the
testis. Supplemental Figure 5 shows all RT=PCR assays used to characterize Ladix. Supplemental Figure 6 displays
IUPred2A output with high probability of disorder across the LAIDX protein.
Supplemental Figure 7 shows dotplots comparing mouse and rat Laidx genes. Supplemental Figure 8 is a
dotplot comparing rat Laidx to the
rat Y chromosome. Supplemental Figure 9 contains dotplots showing similarity of
rat Laidx to a Clonorchis gene. Supplemental Figure 10 displays Periodic-acid
Schiff stained testis histology from WT and Laidx-/Y males demonstrating
normal development and morphology.