Curated chromosome-level assemblies of two Tunisian durum wheat landraces (Triticum turgidum subsp. durum, cv. Chili and Mahmoudi)

This dataset contains the curated chromosome-level assemblies of two Tunisian durum wheat (Triticum turgidum subsp. durum, 2n=4x=28, AABB genome) landraces: Chili and Mahmoudi. It is a refined version of the initial assembly deposit (v1), in which the chromosome-level pseudomolecules were reconstructed by alignment-guided curation against the Svevo.v2 reference to resolve a multi-chromosome scaffold produced during automated Hi-C scaffolding. Four FASTA files are provided: chili_assembly.fsa : Primary scaffold assembly for landrace Chili, generated using hifiasm v0.25.0 and YAHS v1.2a.2 from PacBio HiFi reads with Hi-C scaffolding. Unchanged from the initial deposit. NCBI Assembly accession: DBNSEI000000000. mahmoudi_assembly.fsa : Primary scaffold assembly for landrace Mahmoudi, generated using the same pipeline. Unchanged from the initial deposit. NCBI Assembly accession: DBNSEJ000000000. chili_curated.fasta : Curated chromosome-level pseudomolecules for Chili (14 chromosomes: 1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B, 7A, 7B, plus unplaced scaffolds), reconstructed by alignment-guided curation against the Svevo.v2 reference (IWGSC RefSeq v2.1). mahmoudi_curated.fasta : Curated chromosome-level pseudomolecules for Mahmoudi (same chromosome composition). In the initial assembly, automated Hi-C scaffolding concatenated multiple B-subgenome chromosomes into a single multi-gigabase scaffold (previously reported as a 2B–3B fusion). This mis-join was identified by whole-genome alignment to Svevo.v2: the constituent chromosomes were split at the intervening scaffold gaps, reverse-complemented as required, and ordered against the reference, yielding individual B-subgenome pseudomolecules. The largest chromosome, Chr3B, is 868 Mb, consistent with the expected durum chromosome size. Curated assembly metrics: Chili: total 10.84 Gb; largest chromosome (Chr3B) 858.7 Mb; scaffold N50 756 Mb; 97.1% of the assembly anchored to the 14 chromosomes. Mahmoudi: total 10.70 Gb; largest chromosome (Chr3B) 868.1 Mb; scaffold N50 757 Mb; 97.3% anchored to the 14 chromosomes. Quality metrics (unchanged by curation - base composition, per-base accuracy and k-mer completeness are invariant to scaffold reorganization): Chili: QV 68.0, k-mer completeness 98.42%, BUSCO 99.4% Mahmoudi: QV 68.3, k-mer completeness 98.34%, BUSCO 99.3%