The frequency of in vitro cell transformation by estrogens.
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Anchorage independent growth, an indicator of neoplastic transformation of cells, was assessed as previously described by Zhang et al (31). Briefly, 5000 untreated and estrogen treated cells were seeded in each well and were grown for a minimum of 21 days over agar layer. The frequency of cell transformation was determined by counting the number of colonies that formed in the presence of E2, 4-OH-E2 or 2-OH-E2: (number of colonies formed/the total number of seeded cells) x 100.
创建时间：
2015-12-02
