SNP discovery in wild and domesticated populations of blue catfish, Ictalurus furcatus, using genotyping-by-sequencing and subsequent SNP validation

The SNPs were called by TASSEL 4.0 after mapping the GBS raw reads to the blue catfish genome by BWA. The VCF file holding SNP information that retains information on depth of coverage for each allele, contains all raw SNP calls.将GBS（基因型分型测序，Genotyping-by-Sequencing）原始测序读段通过BWA（Burrows-Wheeler Aligner）软件比对至蓝鲶基因组后，研究人员使用TASSEL 4.0软件完成了单核苷酸多态性（Single Nucleotide Polymorphisms，SNPs）的分型调用。包含所有原始SNP分型调用结果的VCF（变异检测格式，Variant Call Format）文件，保留了每个等位基因的测序覆盖深度信息。