In the presence of rmMCP-6 and rmMCP-7 SVEC4-10 cells show a differential release of angiogenesis related cytokines/proteins.

The graph shows the cytokines/proteins that were released exclusively by rmMCP-6 (blue) or rmMCP-7 (red). Four cytokines/proteins that were released by control cells were increased in the presence of the tryptases (green). The cells were cultured for 5 hours at 37°C on Geltrex® in the presence of rmMCP-6, rmMCP-7 or in the absence of tryptases. After incubation, a mouse cytokine array kit was used to analyze the protein expression of different pro- and anti-angiogenic factors in the culture supernatants. The data shown is the mean spot pixel density that was quantified from the arrays using the image analysis software Adobe Photoshop CS6 V 13.0.