miR-10a-3p as a potential non-invasive biomarker for NSCLC patients' survival

Lung cancer is the most currently diagnosed cancer type among adults and the most common cause of death from cancer worldwide. Poor lung cancer patients' outcomes and survival rates demand discovery of new biomarkers for the specific, significant, and non-invasive detection of non-small cell lung cancer (NSCLC) progression. Determination of cell lines' relationship to genomic changes in tumors could be valuable for functional and therapeutic discoveries. Human cell line models with different aggressiveness statuses may play an important role in the investigation of NSCLC. In addition, miRNA expression patterns that are reliable in predicting NSCLC patients' survival rates are highly promising cancer biomarkers. The aim of the present study was to investigate the potential of miRNA expression as biomarkers in NSCLC. Overall design: In order to determine miRNA expression pattern related to lung cancer aggressiveness total RNA were extracted from HBEC3, H1299, A549, Calu-1, and H23 cultured as multicellular spheroids for miRNA sequencing.