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Effect of FePro- and Endorem-labelling on rat ST14A neural progenitor cell and neurosphere viability and growth in response to ischaemia in vitro

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f1000.figshare.com2023-05-31 更新2025-03-21 收录
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MTT assay file legend:MTT ((3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) colorimetric viability assay of ST14A rat neural progenitor cells treated with Endorem or FePro MRI contrasting agents compared to control. Values are absorbance at 560nm. Alamar blue ST14A legend: Alamar blue colorimetric viability assay of ST14A rat neural progenitor cells treated with Endorem or FePro MRI contrasting agents compared to control. Values are absorbance at 560nm. Alamar blue NPC legend: Alamar blue colorimetric viability assay of rat neurospheres treated with Endorem or FePro MRI contrasting agents compared to control. Values are absorbance at 560nm. Trypan blue NPC legend: Trypan blue viability assay of rat neurospheres treated with Endorem or FePro MRI contrasting agents compared to control. Number of viable cells is given as a percentage. Neuosphere diameter legend: Neurosphere diameter assay to assess cellular proliferation of rat neurospheres treated with Endorem or FePro MRI contrasting agents compared to control. Values given in um2. Neuosphere growth legend: Neurosphere growth assay to assess cellular proliferation of rat neurospheres treated with Endorem or FePro MRI contrasting agents compared to control. Values given as the number of neurpspheres formed per 1000 cells.

MTT 测定文件图例:MTT ((3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴化物) 彩色比色法检测 ST14A 大鼠神经祖细胞经 Endorem 或 FePro MRI 对比剂处理后与对照相比的存活率。数值为 560nm 处的吸光度。 Alamar blue ST14A 图例:Alamar blue 彩色比色法检测 ST14A 大鼠神经祖细胞经 Endorem 或 FePro MRI 对比剂处理后与对照相比的存活率。数值为 560nm 处的吸光度。 Alamar blue NPC 图例:Alamar blue 彩色比色法检测大鼠神经球经 Endorem 或 FePro MRI 对比剂处理后与对照相比的存活率。数值为 560nm 处的吸光度。 Trypan blue NPC 图例:Trypan blue 存活率检测大鼠神经球经 Endorem 或 FePro MRI 对比剂处理后与对照相比的存活率。给出的是存活细胞数的百分比。 神经球直径图例:神经球直径测定用于评估经 Endorem 或 FePro MRI 对比剂处理的大鼠神经球的细胞增殖。数值以平方微米为单位。 神经球生长图例:神经球生长测定用于评估经 Endorem 或 FePro MRI 对比剂处理的大鼠神经球的细胞增殖。数值以每 1000 个细胞形成的神经球数量给出。
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