Gene expression of differentiated human induced pluripotent stem cells

Gene expression of Human DiPS 1016 SeVA iPSCs and hiPSCs-differentiated into endothelial, vascular smooth muscle or white adipose tissue cells. Overall design: RNA was extracted from Human DiPS 1016 SeVA iPSCs and hiPSCs-differentiated into endothelial, vascular smooth muscle or white adipose tissue cells using the QIAcube and the RNeasy Plus Mini Kit (QIAGEN). RNA quality was ascertained with the TapeStation (Agilent), and only samples with a RIN>9.0 value were used for sequencing. Sequencing libraries were made with the PrepX DNA library kit (Takara bio) using the Apollo system (Takara bio). Quality control for the libraries was performed using the KAPA library quantification kit (Roche) and the TapeStation (Agilent). Sequencing libraries were barcoded, pooled and sequenced on a HiSeq 2500 (Illumina) with single-end 150bp reads. Sequencing output was de-multiplexed, barcodes removed, trimmed for quality, and FastQC was run on all files for quality control. Reads were aligned to human genome assembly GRCh38 using STAR76.