Maternal High-Fructose Intake Activates Myogenic Program In Fetal Brown Fat And Predisposes Offspring to Diet-Induced Metabolic Dysfunctions In Adulthood

We performed RNA-seq analysis for evaluating the effect of maternal high-fructose intake on fetal brown fat development. We find that maternal high-fructose intake induces myogenic signature in fetal brown fat, indicating impairment of brown fat development by maternal high-fructose intake. The pregnant mice were randomly assigned to either sterilized tap water or 20% (w/v) fructose (sigma, F3510) water until delivery. At E18.5, a subset of pregnant mice were killed and the BAT of male fetuses were collected for RNA-seq analysis.