Marine microbial degradation of water-soluble crude oil components by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electrospray ionization negative mode (ESI(-)) direct infusion data

This dataset includes the semi-quantitative direct infusion data generated with a Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electrospray ionization negative mode (ESI(-)) from an experiment on marine microbial degradation of water-soluble polar crude oil components. Crude oil-derived polar compounds are receiving increasing attention due to their higher proportion in weathered oil, higher water solubility and potential harmful environmental impacts. However, these compounds are not amenable to GC-based analytical methods. Current knowledge on the characteristics and weathering patterns of oil-derived polar compounds relies on Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Natural marine bacteria consortia were exposed to crude oil-derived compounds dissolved in seawater in a lab incubation study. Three sets of controls were used: (1) sterile seawater with oil-derived water-soluble compounds; (2) sterile seawater with natural marine bacteria and succinic acid, which was used as a simple carbon source instead of crude oil; and (3) seawater alone with natural marine bacteria. All incubation sets were supplemented with nutrients and kept in dark at room temperature for 14 days. This dataset includes semi-quantitative FT-ICR-MS direct infusion data, which focused on the molecular level chemical composition of the incubation media.本数据集包含一项针对水溶性极性原油组分开展的海洋微生物降解实验所产生的半定量直接进样质谱数据，该数据通过傅里叶变换离子回旋共振质谱（FT-ICR-MS）结合电喷雾电离负模式（ESI(-)）获取。原油衍生极性化合物因在风化原油中占比更高、水溶性更强且具有潜在环境危害，正日益受到学界关注。然而这类化合物无法采用基于气相色谱的分析方法进行检测。目前学界对原油衍生极性化合物的特征与风化模式的认知，均依赖傅里叶变换离子回旋共振质谱技术。在本次实验室培养实验中，天然海洋细菌群落被置于溶解有原油衍生化合物的海水环境中进行培养。本实验共设置三组对照组：（1）添加原油衍生水溶性化合物的无菌海水；（2）添加天然海洋细菌与琥珀酸（以琥珀酸作为替代原油的单一碳源）的无菌海水；（3）仅含有天然海洋细菌的天然海水。所有培养体系均补充了营养盐，并在室温避光条件下培养14天。本数据集包含半定量傅里叶变换离子回旋共振质谱直接进样数据，其聚焦于培养介质的分子级化学组成。