Regulation of pyroptosis in radiation induces mouse intestine cells

Pyroptosis is a recently discovered form of lytic cell death that is characterized by cell swelling and formation of pores and large bubbles on the plasma membrane. We find radiation could induce pyroptosis in human colorectal cancer HCT116 cells, and irradiation induces pyroptosis in mouse normal intestine MODEK cells only after 36 hours and over 8.0 Gy. In view of the significant pyroptosis effect of MODE-K cells, we selected 4Gy and 12Gy, 24h and 48h for experiments. After ONT full-length transcriptome sequencing, the cell pyroptosis model can screen out the differential genes related to cell pyroptosis, which is helpful to further explore the mechanism of pyroptosis of MODE-K cells. Mouse intestine MODEK cells were cultured and divided into four groups, then irradiated with 0Gy/0h (ie.0 Gy at 0 hour), 4Gy/48h, 12Gy/24h and 12Gy/48h, then cells were harvested and performed RNA sequencing.