Additional file 2: of Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates

Table S1. The table is organized in the following spreadsheets: Spreadsheet “All Phosphopeptides”: This table lists all identified phosphopeptides from three independent replicates, via LC-MS/MS. Additional information corresponding to each phosphopeptide is also shown and includes peptide ID# (numerical ID), fraction (enriched; 1 or flowthrough; 2 fractions), Retention time, m/z, charge, measured mass, mass error, score, peptide sequence, modifications (type of PTM), UniProt accession, Phosphosite(s), sequence window, description of protein, use in quantitation (at least 2 unique tryptic peptides were identified for the corresponding protein), ANOVA p-values corresponding to each phosphopeptide identified across the three independent replicates, normalized abundance (phosphopeptide intensity) of the identified phosphopeptides across each replicate, average phosphopeptide intensities of the peptide in the control cells or cells expressing wild type SRMS, fold-change abundance of phosphopeptide intensities in SRMS-expressing cells (SRMS/Control) and Log2-scaled fold-change of SRMS/control values. Spreadsheet “All unique phosphoproteins”: This table lists all the unique phosphoproteins mapped from the phosphopeptides quantified via LC-MS/MS in the Vector and SRMS-expressing cells. Also shown are all associated phosphosites identified for each protein. Spreadsheet “Phosphopeptides p-value ≤ 0.05”: This table lists all phosphopeptides filtered at an ANOVA p-value threshold of 0.05. Also shown is other relevant information corresponding to each phosphopeptide, as in Spreadsheet “All Phosphopeptides”. Spreadsheet “Hyperphosphorylated proteins”: This table lists all the hyperphosphorylated/upregulated proteins identified in the SRMS-regulated phosphoproteome. These proteins were mapped from the hyperphosphorylated/upregulated peptides (Log2 SRMS/Control ≥1.58 and ANOVA p-value ≤0.05). Therefore, only phosphosites identified at Log2 SRMS/Control ≥1.58 and ANOVA p-value ≤0.05, are shown for every protein. Spreadsheet “Hypophosphorylated proteins”: Shown here is the hypophosphorylated/downregulated protein mapped from the hypophosphorylated/downregulated peptide (Log2 SRMS/Control ≤ − 1 and ANOVA p-value ≤0.05) identified in SRMS-expressing cells. (XLSX 745 kb)