Cryo-EM structure determination of the PARP2-QFRD•Nuc165 complex.

A: A representative motion-corrected, low-pass filtered micrograph from dataset 1 (scale bar 50 nm). B: Power spectrum and CTF estimation of the micrograph shown in panel A. C: Local resolution map and histogram of local resolution values of the PARP2-QFRD•Nuc165 complex 3D reconstruction from dataset 1. D: Overall resolution of the PARP2-QFRD•Nuc165 complex 3D reconstruction from dataset 1, as determined by the 0.143 FSC threshold (dotted horizontal line). E: Postprocessing Guinier plot for the above data. F: Euler angle distribution of the 27 889 particles that contributed to the 3D reconstruction of the PARP2-QFRD•Nuc165 complex from dataset 1. G: Flow chart of the initial analysis of dataset 1, picking single nucleosomes. H: Flow chart of the second analysis of dataset 1, picking nucleosome dimers. I: Flow chart of the final analysis of dataset 1, using all micrographs and picking nucleosome dimers with crYOLO using a model trained on this dataset. J: A representative motion-corrected, low-pass filtered micrograph from dataset 2. K: Power spectrum and CTF estimation of the micrograph shown in panel J. L: Local resolution map and histogram of local resolution values of the PARP2-QFRD•Nuc165 complex 3D reconstruction from dataset 2. M: Overall resolution of the PARP2-QFRD•Nuc165 complex 3D reconstruction from dataset 2, as determined by the 0.143 FSC threshold (dotted horizontal line). N: Post processing Guinier plot for the above dataset. O: Euler angle distribution of the 16 304 particles that contributed to the 3D reconstruction of the PARP2-QFRD•Nuc165 complex from dataset 2. P: Flow chart of the final analysis of dataset 2, using all micrographs and picking nucleosome dimers with crYOLO using a model trained on datasets 1 and 2. (RAR)