Mesenchymal stem cells receive adaptive islet-derived exosomal miR-151 to promote ß-cell compensation in obesity

C57/B6J mice were fed with NCD or HFD for 9 weeks. The isolated islets were resuspended and cultured in a serum-sEVs-free medium for 48 hours. The culture medium was collected for sEVs isolation. Total RNA from sEVs were extracted using the miRNeasy Mini Kit (Qiagen, USA). A total of 5 ng RNA was used for miRNA library construction with the NEBNexR UltraTM small RNA Sample Library Prep Kit for IlluminaR (NEB, USA). The library was sequenced using the Illumina NovaSeq 6000 platform. Overall design: miRNA-seq of sEVs derived from NCD and HFD islets.