Two-bottle preference experiments with water and 200mM sucrose before and after treatment with naloxone

Two-bottle preference experiments with water and 200mM sucrose before and after treatment with naloxone. In the first set of experiments in Figure 7A, three mice had continual access to water and sugar solution. The animals were trained for three days before receiving naloxone treatment. For each day, the total amounts of each liquid consumed were used to calculate the preference score [sucrose (mL)/total fluid (mL)] such that the higher the score indicates the greater the sweet preference with 0.5 representing no preference. In the second set of experiments in Figure 7B, four mice were placed on a 22.5 hour water deprivation schedule where they had access to bottles of water and sugar solution the remaining 1.5 hours per day.纳洛酮（naloxone）给药前后，针对水与200mM蔗糖的双瓶偏好实验。在图7A的第一组实验中，3只小鼠可持续获取水与蔗糖溶液。实验动物在接受纳洛酮给药前，先进行了3天的适应性训练。每日以两种液体的总消耗量计算偏好得分[蔗糖体积(mL)/总液体体积(mL)]，得分越高代表对甜味的偏好越强，当得分为0.5时则代表无偏好。在图7B的第二组实验中，4只小鼠被实施22.5小时的饮水剥夺方案，每日仅可在剩余的1.5小时内接触水瓶与蔗糖溶液。