Reversal of human regulatory T cell suppression throgh TLR8 signaling

Analyses of gene alterations of human Treg cells treated with or without TLR8 ligand using the Illumina whole-genome Human HT-12 BeadChips. The study will provide important information for the molecular signature changes during the reversal of human Treg suppression mediated by activation of TLR8 signaling. Human naturally occurring Treg cells were isolated from PBMCs of healthy donors and treated with or without Poly-G3 for 0 and 24 hours. Total RNA was purified from Poly-G3 treated and untreated human Treg cells, and transcriptome analyses of Treg cells were performed using the Illumina whole-genome Human HT-12 BeadChips.