Development and characterization of microsatellite markers for Moricandia moricandioides (Brassicaceae) and cross-amplification in related species. Moricandia Microsatellite

- Premise of the study: Polymorphic microsatellite markers were developed to study population structure and mating patterns of the monocarpic herb Moricandia moricandioides (Brassicaceae)- Methods and results: Illumina MiSeq sequencing was used to develop a panel of 15 polymorphic microsatellite markers that were tested across 77 individuals from three populations on the Iberian Peninsula. All markers were polymorphic in at least two studied populations, and the number of alleles ranged from one to 11 per locus. The observed and expected heterozygosity ranged from 0 to 1.00 and from 0.153 to 0.855, respectively. Nine and 11 loci were successfully amplified in the congeneric species M. arvensis and M. foetida, respectively- Conclusions: The 15 microsatellite markers are useful for population genetic studies of the genus Moricandia. These markers will serve as a useful tool for exploring population structure and mating patterns of M. moricandioides. These microsatellites should also be suitable for studies in other Moricandia species.