Allelic silencing analysis for A-repeat and m6A region in mouse Xist

We show that a deletion encompassing the entire Xist 5' m6A region results in a modest reduction in Xist-mediated silencing. Overall design: We isolated chromatin-associated RNA (ChrRNA) to analyze the allelic ratio, aiming to understand Xist-mediated Silencing. The ChrRNA was isolated from cells iXist-ChrX derived from mouse embryonic stem cells. Xist is composed of several repetitive elements, which act by interacting with different factors. Cell lines with Xist domain deletions were generated. The allelic ratios were systematically and comprehensively compared.