Coomassie-stained gels used in semi-quantitative analysis of purified calcineurin from calmodulin Sepharose resins.

Coomassie-stained gels used for a semi-quantitative analysis of purified calcineurin from novel calmodulin Sepharose resins. The gel images depict the alpha and beta subunits of calcinuerin (at 60kDa and 19kDa, respectively) in 3 peak elution fractions from two types of CaM affinity resins, as well as BSA and Lysozyme (66kDa and 14kDa, respectively) at varying concentrations (mg/mL) that were used to create a standard curve for calculating the amount of total protein obtained in each purification.