Sources of B. distachyon (2n = 10), B. stacei (2n = 20) and B. hybridum (2n = 30) samples analysed for the trnLF, ITS and GI loci.

Asterisks indicate chromosome counts obtained in this study using DAPI-staining methods. Other chromosome numbers and/or ploidy levels were retrieved from the literature or have been provided by the germplasm collectors and curators (see abbreviations below). For each sample, the sequences have been classified into the corresponding trnLF, ITS and GI haplotypes (see Table S2). Bdis and Bsta indicate B. distachyon-type and B. stacei-type sequences, respectively. c: cloned sequences; n.a: not amplified. Abbreviations of germplasm and sample collectors and curators, germplasm banks and herbaria: AB & SE: Adina Breiman and Smadar Ezrati; ABR: Aberystwyth (UK); AC: Ana Caicedo; AM: Antonio Manzaneda; DL & PC: Diana López-Alvarez & Pilar Catalán; EB: Elena Benavente; CS: Consuelo Soler; Hikmet Budak: HB; INIA-CRF: Instituto Nacional de Investigaciones Agrarias - Centro de Recursos Fitogenéticos (Spain); INRA: Institut National de la Recherche Agronomique (France); JV: John Vogel; MA: Real Jardin Botanico de Madrid herbarium; Metin Tuna: MT; MRR: Mohammad Reza Rahiminejad; PC & LM: Pilar Catalan and Luis Mur; RH-JA: (Univ.) Reading Herbarium - Joel Allainguillaume; SH: Samuel Hazen; ST: Salvador Talavera; USDA: United States Department of Agriculture. Literature references for chromosome counts and ploidy levels: Jenkins et al. (2003), Filiz et al. (2009); Catalán et al. (2012), Giraldo et al. (2012); Hammami et al. (2011), Manzaneda et al. (2011); Mur et al. (2011); Vogel et al. (2009).