Differentially Expressed Genes (DEGs) in floral bud transcriptomes

The relative abundance for each transcript and unigene was quantified in the floral bud samples (S1, flowering induction; S2-S4, flower whorls development and D, dormancy) by aligning the reads to the transcriptome assembly using RSEM v1.3.0 software. To identify the differentially expressed genes the Deseq2 1.12.4 software was used. <br>The Summary of DEGs.xlsx archive contains: the "Tables sheet" with tables about the number of DEGs (up- and down-regulated) in sucessive pairwise comparisons of floral buds, GO summary and the number and percentaje of DEGs codifying transcription factors; the "Gene Annotation sheet" with the annotation of DEGs; the Gene Ontology sheet" with the GO terms found for each transcript; the "KEGG sheet" with the KEGG terms found for each transcript; the "Transcription Factor Families" sheet with the description of DEGs codifying transcription factors.The DEGs.fasta archive contains the deduced nucleotide sequences of DEG transcripts.The diff.Expr.matrix... archive contains a heatmap showing the log2 of fold-change of DEGs vs the samples analized (S1-S4 and D floral buds). Yellow and magenta colors represent up- and down-regulated genes, respectively. Scale, representing the signal values, is shown at the top of the figure.<br>The "MADS-box transcripts found during floral bud development in P. avium cv. Bing”.xlsx archive includes the Trinity identification (Trinity #id), MADS-box gene identification (Gene ID, this work), Gene Subfamily, Genbank accession number considering only <i>P. avium</i>, mean value of Transcripts Per Million (TPM) in S1-S4 and Dormancy (D) buds, Translation (Complete/Partial) using TransDecoder, Diferential expression (DEG Yes/No) and if the MADS-box genes was analyzed by qPCR.