Single cell RNA-sequencing on E17.5 mouse lungs and tracheas

To identify clear mesenchymal cell clusters, sequencing libraries of embryonic lung cells were prepared using the 10x Genomics Chromium system. After quality control samples were integrated. The cells were visualized in two dimensions according to their gene expression profiles using Uniform Manifold Approximation and Projection (UMAP). We FACS sorted live cells from three E17.5 murine lungs and tracheas. Library preparation and single cell RNA-seq was performed on purified single cells.