RNAseq of GL261 tumor endothelial cells from wild type and Eltd1-/- mice

Eltd1 is widely expressed in endothelial cells. We have reported that the expression of Eltd1 is highly up-regulated in glioma vessels. Other studies have shown that Eltd1 is up-regulated in vessels in other types of tumors, and has been suggested as a vascular target for anti-angiogenesis therapy. However, the role of Eltd1 in vessels is largely unknown. We isolated endothelial cells from healthy brain tissue and GL261 tumor tissue, and analyzed the role of Eltd1 in tumor angiogenesis by analyzing the endothelial gene profile from wild type and Eltd1-/- mice. We injected GL261 intracranially to C57BL/6 wild type and Eltd1-/- mice. Tumors were dissected under a stereological microscope. To isolate endothelial cell mRNA from heathy brain tissue and tumor tissue, we took advantage of the mouse that expressing a fusion eGFP-L10a ribosomal protein under control of the VE-cadherin promoter, and crossed it with the Eltd1-/- mouse. Using translating ribosome affinity purification (TRAP) strategy, endothelial specific mRNA was isolated and were further prepared for RNAseq analysis.