Transcriptomic data of BT549 triple negative breast cancer cells treated with 20 μM NU7441,a DNA-dependent kinase inhibitor

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a multifunctional serine-threonine protein kinase that plays pleiotropic roles in cancer.NU7441 is a specific DNA-PKcs inhibitor.We investigated the effect of the DNA-dependent kinase (DNA-PK) inhibitor, NU7441, on the transcriptome of BT549 triple negative breast cancer (TNBC) cells.Total RNA extracted from NU7441 or vehicle-treated cells was processed for preparation of sequencing libraries . Assessment of read quality was performed using fastqc tool. Trimming and filtering low-quality reads were performed using fastp. Reads were aligned by hisat2. SAM files were converted to BAM files using Samtools. Mapped reads were quantified using featureCounts.The RNA sequencing results were also subjected to gene differential expression analysis, Gene Ontology (GO) analysis and KEGG pathway analysis. After NU7441 treatment, total number of 2045 differential genes were selected according to |log2(FoldChange)| >= 1 & padj<= 0.05, among which 1365 genes were down-regulated and 680 genes were up-regulated. The differential genes in pattern recognition receptors (PRRs) immune responses signals, including NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and Cytosolic DNA-sensing pathways were noted in this paper .

DNA依赖性蛋白激酶催化亚基（DNA-PKcs）是一种多功能丝氨酸/苏氨酸蛋白激酶，在癌症中发挥着多效作用。NU7441是一种针对DNA-PKcs的特异性抑制剂。本研究旨在探究DNA依赖性激酶（DNA-PK）抑制剂NU7441对BT549三阴性乳腺癌（TNBC）细胞转录组的影响。从经NU7441或对照处理后的细胞中提取总RNA，用于制备测序文库。采用fastqc工具对测序读段质量进行评估，并使用fastp进行剪接和过滤低质量读段。利用hisat2进行读段对齐，通过Samtools将SAM文件转换为BAM文件。利用featureCounts对映射读段进行量化。RNA测序结果亦进行了基因差异表达分析、基因本体（GO）分析和KEGG通路分析。在NU7441处理后，根据|log2（变化倍数）|≥1且校正p值≤0.05的标准，共筛选出2045个差异基因，其中1365个基因表达下调，680个基因表达上调。本文还关注了模式识别受体（PRRs）免疫反应信号中的差异基因，包括NOD样受体信号、Toll样受体信号、RIG-I样受体信号和细胞质DNA感受通路。