Table_1_Association Between Circular RNAs and Intracranial Aneurysm Rupture Under the Synergistic Effect of Individual Environmental Factors.doc

Introduction: To study the association between specific circular RNAs and rupture of intracranial aneurysm. To explore its clinical diagnostic significance and synergistic effects with individual environmental influencing factors.Methods: Three hundred and forty seven cases and controls were included in this study. Multivariate analysis was used to explore the main individual environmental factors. Intracranial aneurysm rupture related circular RNAs screened based on sequencing was verified in peripheral blood by PCR. ROC curve, logistic regression model and fork analysis were used to study the association, diagnostic values, and synergistic effects of circular RNA with intracranial aneurysms and individual environmental factors.Results: Smoking, hair dyeing, sitting time ≥6 h/day, single animal oil intake and hypertension are the main risk factors for intracranial aneurysm rupture; People with higher education, sleeping time ≥7 h/day, tea drinking, diabetes, higher levels of (hemoglobin, low density lipoprotein, serum calcium, and apolipoprotein-A1) have a low risk of intracranial aneurysm rupture. Hsa_circ_0008433 and hsa_circ_0001946 are closely related to intracranial aneurysm rupture and have certain clinical diagnostic significance (AUC = 0.726; 95% CI: 0.668~0.784). Hsa_circ_0008433 (OR = 0.497, 95% CI: 0.338~0.731), hsa_circ_0001946 (OR = 0.682, 95% CI: 0.509~0.914) were independent epigenetic factors affecting intracranial aneurysm rupture, and have a multiplicative interaction with age (OR = 3.052, 95% CI: 1.006~9.258).Conclusions: Low expressions of hsa_circ_0008433 and hsa_circ_0001946 are risk factors for intracranial aneurysms rupture and have good clinical diagnostic value. There was a multiplicative interaction between epigenetic score and age. The older and the higher the epigenetic score was, the more likely to have intracranial aneurysm rupture.

引言：本研究旨在探讨特定环状RNA与颅内动脉瘤破裂之间的关联，并探究其临床诊断意义及其与个体环境影响因素的协同作用。方法：本研究纳入了347例病例组和对照组。通过多元分析探究主要的个体环境因素。基于测序筛选的颅内动脉瘤破裂相关环状RNA通过PCR在周围血液中进行验证。采用ROC曲线、逻辑回归模型和分支分析研究环状RNA与颅内动脉瘤及个体环境因素之间的关联、诊断价值和协同作用。结果：吸烟、染发、每日静坐时间≥6小时、单动物油摄入和高血压是颅内动脉瘤破裂的主要风险因素；受教育程度较高、每日睡眠时间≥7小时、饮茶、糖尿病以及（血红蛋白、低密度脂蛋白、血清钙和载脂蛋白A1）水平较高的人群颅内动脉瘤破裂风险较低。Hsa_circ_0008433和Hsa_circ_0001946与颅内动脉瘤破裂密切相关，并具有一定的临床诊断意义（AUC = 0.726；95% CI: 0.668~0.784）。Hsa_circ_0008433（OR = 0.497，95% CI: 0.338~0.731）、Hsa_circ_0001946（OR = 0.682，95% CI: 0.509~0.914）是影响颅内动脉瘤破裂的独立表观遗传学因素，并与年龄具有乘法交互作用（OR = 3.052，95% CI: 1.006~9.258）。结论：Hsa_circ_0008433和Hsa_circ_0001946的低表达是颅内动脉瘤破裂的风险因素，并具有良好的临床诊断价值。表观遗传学评分与年龄之间存在乘法交互作用。表观遗传学评分越高、年龄越大，颅内动脉瘤破裂的风险越高。