Stat5 induces androgen receptor (AR) gene transcription in prostate cancer and offers a druggable pathway to target androgen receptor signaling

The goal of this study was to compare the global transcriptomic effects of knockdown of AR vs inhibition of STAT5 via treatment with IST5-002 in the prostate cancer cell line CWR22Rv1. We knocked down AR with 2 independent shRNAs (or shCtrl). We treated cells for 72 hrs with 0.8 µM IST5-002 (or DMSO control). Comparative gene expression profiling in CWR22Rv1 cells between five groups total. CWR22Rv1 cells with AR knocked down via shRNA (shAR-574, shAR-847 and control shCtrl) or treated for 72 hours with 0.8 uM IST5-002 (or DMSO control).