Bessa-Gonçalves et al2021_Cytokine array data

The hypothesis underlying this data was that modifying Fibrinogen (Fg) scaffolds with Magnesium (Mg) incorporation could modulate macrophage polarisation towards M2 anti-inflammatory phenotype, in vitro and in vivo. Cytokine array data are provided for: - Human cytokine array. Cytokines present in conditioned media from macrophages cultured, for 13 days, in Fg and FgMg (10 mM) scaffolds in the presence/absence of M1 stimulation (LPS+INFg) were quantified at Eve Technologies Corp. (Calgary, AB, Canada) using the Human Cytokine Array/Chemokine Array 48-Plex: soluble CD40 ligand (sCD40L), epidermal growth factor (EGF), Eotaxin/CCL11, fibroblast growth factor 2 (FGF-2), FMS-related tyrosine kinase 3 ligand (Flt-3 ligand), Fractalkine/CX3CL1, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), growth-regulated on alpha protein (GRO-⍺/CXCL1), interferon alpha-2 (IFN⍺2), interferon gamma (IFN𝛄), interleukin (IL)-1⍺, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, interferon induced protein 10 (IP-10/CXCL10), monocyte chemoattractant protein (MCP)-1/CCL2, MCP-3/CCL7, macrophage colony-stimulating factor (M-CSF), macrophage-derived chemokine (MDC/CCL22), monokine induced by interferon gamma (MIG/CXCL9), macrophage inflammatory protein -1 alpha (MIP-1⍺/CCL3), macrophage inflammatory protein -1 beta (MIP-1β/CCL4), platelet derived growth factor (PDGF)-AA, PDGF-AB/BB, regulated upon activation, normal T cell expressed, and secreted (RANTES/CCL5), transforming growth factor alpha (TGF⍺), tumor necrosis factor (TNF)⍺, TNFβ and vascular endothelial growth factor A (VEGF-A). The sensitivity of the assay for the analysed markers ranged from 0.14 to 50.78 pg/mL. - Mouse cytokine array. Cytokines present in inflammatory exudates were quantified at Eve Technologies Corp. (Calgary, AB, Canada) using the using the Mouse Cytokine Array/Chemokine Array 44-Plex: Eotaxin/CCL11, Erythropoietin, 6Ckine/CCL21, Fractalkine/CX3CL1, G-CSF, GM-CSF, interferon beta 1 (IFNB1), IFN𝛄, IL-1⍺, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, keratinocyte chemoattractant (KC), leukemia inhibitor factor (LIF), lipopolysaccharide-induced CXC chemokine (LIX/CXCL5), MCP-1/CCL2, MCP-5/CCL12, M-CSF, MDC/CCL22, MIG/CXCL9, MIP-1⍺/CCL3, MIP-1β/CCL4, MIP-2/CXCL2, MIP-3⍺/CCL20, MIP-3B/CCL19, RANTES, thymus- and activation-regulated chemokine (TARC/CCL17), tissue inhibitor of metalloproteinase (TIMP)-1, TNFα, and VEGF. The sensitivity of the assay for the analysed markers ranged from 0.1 to 33.3 pg/mL. Both data for a pilot run (performed to determine if sample concentration was appropriate) and a run for all the other samples are provided. The sample identification legend is provided in all the files.本数据集所依托的研究假说为：在体外与体内环境中，通过掺入镁（Magnesium, Mg）对纤维蛋白原（Fibrinogen, Fg）支架进行修饰，可调控巨噬细胞极化为M2型抗炎表型。本次提供的细胞因子阵列数据分为两部分： - 人类细胞因子阵列数据：于加拿大艾伯塔省卡尔加里的Eve Technologies Corp.实验室，采用人类细胞因子/趋化因子48重阵列（Human Cytokine Array/Chemokine Array 48-Plex），对分别于纤维蛋白原支架、掺入10 mM镁的纤维蛋白原（FgMg）支架中，在有/无M1刺激（脂多糖LPS+干扰素γINFγ）的条件下培养13天的巨噬细胞条件培养基中的细胞因子进行定量检测。所检测的细胞因子包括：可溶性CD40配体（soluble CD40 ligand, sCD40L）、表皮生长因子（epidermal growth factor, EGF）、嗜酸细胞趋化蛋白/CCL11（Eotaxin/CCL11）、成纤维细胞生长因子2（fibroblast growth factor 2, FGF-2）、FMS相关酪氨酸激酶3配体（FMS-related tyrosine kinase 3 ligand, Flt-3配体）、趋化因子Fractalkine/CX3CL1、粒细胞集落刺激因子（granulocyte colony-stimulating factor, G-CSF）、粒细胞-巨噬细胞集落刺激因子（granulocyte-macrophage colony-stimulating factor, GM-CSF）、α型生长调节蛋白（growth-regulated on alpha protein, GRO-α/CXCL1）、α干扰素-2（interferon alpha-2, IFNα2）、γ干扰素（interferon gamma, IFNγ）、白细胞介素（IL）-1α、IL-1β、IL-1受体拮抗剂（IL-1ra）、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12（p40亚基）、IL-12（p70亚基）、IL-13、IL-15、IL-17A、IL-17E/IL-25、IL-17F、IL-18、IL-22、IL-27、干扰素诱导蛋白10（interferon induced protein 10, IP-10/CXCL10）、单核细胞趋化蛋白（MCP）-1/CCL2、MCP-3/CCL7、巨噬细胞集落刺激因子（macrophage colony-stimulating factor, M-CSF）、巨噬细胞来源趋化因子（macrophage-derived chemokine, MDC/CCL22）、干扰素γ诱导单核因子（monokine induced by interferon gamma, MIG/CXCL9）、巨噬细胞炎症蛋白-1α（macrophage inflammatory protein -1 alpha, MIP-1α/CCL3）、巨噬细胞炎症蛋白-1β（macrophage inflammatory protein -1 beta, MIP-1β/CCL4）、血小板衍生生长因子（platelet derived growth factor, PDGF）-AA、PDGF-AB/BB、活化正常T细胞表达与分泌调节因子（regulated upon activation, normal T cell expressed, and secreted, RANTES/CCL5）、转化生长因子α（transforming growth factor alpha, TGFα）、肿瘤坏死因子（TNF）α、TNFβ以及血管内皮生长因子A（vascular endothelial growth factor A, VEGF-A）。该检测方法对上述分析标志物的灵敏度范围为0.14~50.78 pg/mL。 - 小鼠细胞因子阵列数据：同样于Eve Technologies Corp.实验室，采用小鼠细胞因子/趋化因子44重阵列（Mouse Cytokine Array/Chemokine Array 44-Plex），对炎症渗出液中的细胞因子进行定量检测。所检测的细胞因子包括：Eotaxin/CCL11、促红细胞生成素（Erythropoietin）、6Ckine/CCL21、Fractalkine/CX3CL1、G-CSF、GM-CSF、β干扰素1（interferon beta 1, IFNB1）、IFNγ、IL-1α、IL-1β、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-9、IL-10、IL-11、IL-12（p40亚基）、IL-12（p70亚基）、IL-13、IL-15、IL-16、IL-17、IL-20、IP-10、角质形成细胞趋化因子（keratinocyte chemoattractant, KC）、白血病抑制因子（leukemia inhibitor factor, LIF）、脂多糖诱导CXC趋化因子（lipopolysaccharide-induced CXC chemokine, LIX/CXCL5）、MCP-1/CCL2、MCP-5/CCL12、M-CSF、MDC/CCL22、MIG/CXCL9、MIP-1α/CCL3、MIP-1β/CCL4、MIP-2/CXCL2、MIP-3α/CCL20、MIP-3B/CCL19、RANTES、胸腺活化调节趋化因子（thymus- and activation-regulated chemokine, TARC/CCL17）、基质金属蛋白酶抑制剂（tissue inhibitor of metalloproteinase, TIMP）-1、TNFα以及VEGF。该检测方法对上述分析标志物的灵敏度范围为0.1~33.3 pg/mL。 本数据集同时提供预实验（用于验证样本浓度是否适配）以及其余所有样本的检测数据，所有数据文件均附带样本标识图例。