Effect of Vitamin D Modulation on the TH2 Cell Transcriptomic Landscape

This dataset examines the transcriptional effects of vitamin D deficiency in TH2 cells isolated from Mus musculus (C57BL/6 strain). Vitamin D-deficient mice were bred and maintained under UV-free conditions with a vitamin D-depleted diet. TH2 cells from these mice (N=7) were compared to TH2 cells from wild-type mice (N=3) to assess the impact of chronic vitamin D deficiency on gene expression. RNA-seq was used to generate raw gene-level counts. Overall design: This study explores transcriptional changes in TH2 cells from vitamin D-deficient versus wild-type mice. Naïve CD4+ T cells were isolated, polarized into TH2 cells, and analyzed using RNA-seq. Differential expression analysis was performed to identify genes and pathways affected by vitamin D deficiency.