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Identification of toxic proteins encoded by mycobacteriophage TM4 using a next generation sequencing-based method

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NIAID Data Ecosystem2026-03-14 收录
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We describe a next-generation sequencing (NGS)-based, high throughput screening approach for the identification of mycobacteriophage-encoded proteins that are toxic to mycobacteria. A plasmid derived library representing the mycobacteriophage TM4 genome was constructed and transformed into Escherichia coli and Mycobacterium smegmatis.

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2022-12-03
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