Comparison of gene expression profile among four groups of mice that developed under the different combination of genes and environments

To investigate the mechanisms underlying the emergence of schizophrenic phenotypes, especially in sucrose fed Glo1 heterozygote mice, we conducted a transcriptome analysis of PFC regions using microarray experiments among the four groups of mice. The coagulation factor V, which is an essential participant in the processes of production of fibrin from fibrinogen, ranked seventh on the list of transcripts that exhibited more than doubled expression levels in sucrose fed Glo1 heterozygote mice compared with the remaining three groups. This result supports our idea that angiopathy with fibrrin deposition is a novel phenotype in schizphrenia. We investigated four groups of mice that were fed two kinds of diets for about 70 days immediately after weaning (from postnatal day 21): wild-type, starch-fed mice (control, CTL); wild-type, sucrose-fed mice; Glo1 heterozygous, starch-fed mice; and Glo1 heterozygous, sucrose-fed mice. Three independent PFC samples were mixed in each group of mice.