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Mass spectrometry identification of proteins binding to ac4C-modified HBV RNA

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Biotinylated in vitro transcribed HBV pgRNA fragments (with 0%, 50%, or 100% ac4C incorporation) were incubated with HepG2.2.15 cell lysates. Pull-down fractions were analyzed by LC-MS/MS to identify proteins that specifically bind to ac4C-modified RNA.

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2026-06-30
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