B73 maize total leaf proteome under a heat stress time-course

A heat stress treatment was imposed on B73 inbred maize three weeks after germination by increasing ambient air temperatures in the greenhouse from 25C to 30C for one day followed by 40C for 7 days, before a recovery phase at 25C for 3 days. We developed a broad-level view of heat stress effects by determining the quantitative total leaf proteome at the reference unstressed time-point (0 days heat stress; 25C), the peak heat stress time-point (7 days heat stress), and the recovery time point (3 days heat recovery).