River channel connectivity shifts metabolite composition and dissolved organic matter chemistry

This excel workbook contains sheets with data obtained from surface water and hyporheic samples that were were collected from two subalpine streams in Rocky Mountian National Park, Colorado, USA. We collected surface and hyporheic water samples to monitor changes in fluvial chemistry through space (spanning 3 km of valley length at two watersheds with river networks of differing geomorphic complexity) and time (eight time points from high to low river flows). We selected two river segments (‘simple network’ and ‘complex network’), with nearly identical climate regimes and underlying geology, that differ substantially in channel complexity. We sampled three sites within the simple network and seven sites within the complex network (including within an active multi-thread beaver meadow complex (MS and SS), a beaver pond (BP1) that lacks surface water connectivity to the main channel, and confined upstream (UC) and downstream (LC) simple morphology river segments). We used complementary approaches, including EI GC-MS and excitation-emission fluorescence spectroscopy (EEMS Regions I through V), to quantify spatial and temporal differences in DOM molecular composition.<br><b>Data description:</b><br><br><b>AlpineSteam.mspLib</b> contains the EI GC-MS dataset<br><b>Complete_Dataset.xlsx </b>has three tabs:<br><i>1. Metadata: </i>provides the variable names, statistical IDs, and list of annotated metabolites<br><i>2. EI GC-MS: </i>normalized EI GC-MS data used for ordinations<br><i>3. Mass Fragments: </i>clustered spectrum of ion fragments for each TMS-derived metabolite, reporting the absolute ion intensity and the quasi-molecular ion <i>m/z </i>values. <br>Data Citation: Laurel M. Lynch, Nicholas A. Sutfin, Timothy S. Fegel, Claudia M. Boot, Timothy P. Covino, Matthew D. Wallenstein. River channel connectivity shifts metabolite composition and dissolved organic matter chemistry.