EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods

This study includes experiments from the following five sequencing strategies: 1) Shotgun DNA sequencing; 2) Amplicon sequencing after 18S amplification by PCR using 1391F/EukB primer set. Library were constructed according to Illumina Library protocol without any sizing; 3) Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol without any sizing; 4) Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol with a sizing step selecting the 450-650 bp fragments; and 5) Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol with a sizing step selecting the 650-850 bp fragments.本研究包含以下五种测序策略相关的实验：1) 霰弹枪DNA测序（Shotgun DNA sequencing）；2) 使用1391F/EukB引物对通过PCR扩增18S rRNA基因后进行扩增子测序（Amplicon sequencing），文库构建遵循Illumina文库构建流程，未进行片段大小筛选步骤；3) 使用515F/926R引物对通过PCR扩增16S rRNA基因后进行扩增子测序，文库构建遵循Illumina文库构建流程，未进行片段大小筛选步骤；4) 使用515F/926R引物对通过PCR扩增16S rRNA基因后进行扩增子测序，文库构建遵循Illumina文库构建流程，且包含片段大小筛选步骤，筛选450-650 bp的DNA片段；5) 使用515F/926R引物对通过PCR扩增16S rRNA基因后进行扩增子测序，文库构建遵循Illumina文库构建流程，且包含片段大小筛选步骤，筛选650-850 bp的DNA片段。