Analysis of microsatellite loci in tree of heaven (Ailanthus altissima (Mill.) Swingle) using SSR-GBS

Microsatellite markers are still the marker of choice for many research questions in the field of forest genetics. However, the number of available markers is often low for species that have not been studied intensively like the tree of heaven (Ailanthus altissima). During the last decade, next generation sequencing (NGS) has offered advanced techniques for efficiently identifying microsatellite markers and accurately genotyping samples. Here, we identify new microsatellite markers for the tree of heaven by applying an NGS-based method using the Illumina MiSeq platform. NGS technology was proved to be an effective method for fast and cost-efficient identification of microsatellite markers by implementing a genotyping-by-sequencing approach based on Illumina amplicon sequencing (SSR-GBS). We screened three populations from Eastern Austria for genetic variation at 19 newly identified microsatellite loci. We tested two different genotyping approaches: (1) considering only allele lengths (f...微卫星标记（microsatellite markers）仍是森林遗传学领域诸多研究问题的首选分子标记。然而，对于臭椿（Ailanthus altissima）这类尚未被深入研究的物种而言，可用的标记数量往往较为有限。近十年来，下一代测序（next generation sequencing, NGS）技术已提供了高效鉴定微卫星标记并精准完成样本基因分型的先进手段。本研究借助基于Illumina MiSeq平台的NGS方法，为臭椿开发了全新的微卫星标记。通过采用基于Illumina扩增子测序的测序分型技术（SSR-GBS），研究证实NGS技术是实现快速且经济高效地鉴定微卫星标记的有效手段。本研究针对奥地利东部的3个种群，在19个新鉴定的微卫星位点上筛选了遗传变异情况。本研究还测试了两种不同的基因分型方法：(1) 仅考虑等位基因长度（f...