Widely targeted LC-ESI-MS/MS metabolomics of NK-92 cells: IgG versus anti-TIGIT in Mtb-infected macrophage co-culture

Widely targeted LC-ESI-MS/MS metabolite quantification for sorted NK-92 cells after 24 h co-culture with Mycobacterium tuberculosis-infected THP-1 macrophages, following pretreatment with IgG isotype control (IgG-1 to IgG-3) or anti-TIGIT blocking antibody (TIGIT-1 to TIGIT-3). n = 3 per group. The platform used was an ExionLC AD UPLC coupled to a QTRAP 6500+ mass spectrometer (SCIEX), with profiling and annotation performed by MetWare (Wuhan, China). The dataset includes two files: 00_sample_group.csv, a sample-to-group lookup, and 01_metabolite_quantification_matrix.csv, a matrix of 477 compounds across 6 samples with HMDB identifiers and compound classes, of which 220 compounds were quantified in at least one sample (N/A = not detected / below the limit of quantification). These data underlie Fig. 2A, B and the metabolite-anchored integrative analysis (Fig. 2D, E) of the manuscript "A lactate-TBX21-TIGIT axis in glutamine-dependent mitochondrial dysfunction of NK cells during tuberculosis".