RNA-seq analyses of human periodontal ligament fibroblasts (PDLF) transfected with siRNA for PPARg

Purpose : This study was performed to investigate the effects of knockdown of PPARg for osteo/cementogenic differentiation of PDLF through RNA sequencing. Method : PDLF were transfected wtih either control siRNA or siRNA for PPARg and then cultured in mineralization inducing medium for 6 days. Total RNAs were collected before and after the culture. Result : Gene ontology analyses of RNA-seq data revealed the genes functionally related with ECM such as collagen synthesis and trimerization, proteoglycans, and fibers were selectively increased by 6-day culture and suppressed biological processes by si-PPARg compared with si-control at day 6 exhibited 5 of 7 upper ranked biological processes were related with ECM organization and metabolism. Moreover, the upper rank of suppressed pathways by si-PPARg compared with si-control at day 6 showed `Endochondral Ossification` was ranked 1st. Conclusions:These results suggested that knockdown of PPARg was sufficient to eliminate osteo/cementogenic and ECM organizing potentials by comprehensive transcriptional dysregulation in PDLF. Overall design: Examination of the gene expression changes during long-term culture and also between control siRNA- and si-PPARg-transfected PDLF.