Adhesive and mechanical properties of the glue produced by 25 Drosophila species

Drosophila glue, a bioadhesive produced by fly larvae to attach themselves to a substrate for several days, has recently gained attention for its peculiar adhesive and mechanical properties. Although Drosophila glue production was described more than 50 years ago, a general survey of the adhesive and mechanical properties of this proteinaceous gel across Drosophila species is lacking. To measure adhesion, we present here a protocol that is robust to variations in protocol parameters, pupal age and calculation methods. We find that the glue, which covers the entire pupal surface, increases the animal rigidity and plasticity when bound to a glass slide. Our survey of pupal adhesion in 25 Drosophilidae species reveals a wide range of phenotypes, from species that produce no or little glue and adhere little, to species that produce high amounts of glue and adhere strongly. One species, D. hydei, stands out from the rest and emerges as a promising model for the development of future bioadhes..., , , # Dataset for \"Adhesive and mechanical properties of the glue produced by 25 Drosophila species\"

[https://doi.org/10.5061/dryad.79cnp5j3j](https://doi.org/10.5061/dryad.79cnp5j3j)

This dataset comprises all the supporting files associated with the manuscript \"Adhesive and mechanical properties of the glue produced by 25 Drosophila species\" by Monier et al.

## Description of the data and file structure

### File S1.
FileS1\_dorsal\_views\_light\_pupae.zip (1.99 GB).Dorsal views of light pupae. Compressed zip file of 1178 pupae pictures from the dorsal view (.tiff and .jpg). This file contains pictures of pupae manually selected for their light pupal case colour. The name of each image corresponds to the single pupa id, which is the date of adhesion test in format YYYYMMDD followed by a two-digit number which corresponds to the order in which pupa were processed on a given day. To find the corresponding species and associated information, please check Table “metadata_concatenate.csv” ...