Adhesive and mechanical properties of the glue produced by 25 Drosophila species

Drosophila glue, a bioadhesive produced by fly larvae to attach themselves to a substrate for several days, has recently gained attention for its peculiar adhesive and mechanical properties. Although Drosophila glue production was described more than 50 years ago, a general survey of the adhesive and mechanical properties of this proteinaceous gel across Drosophila species is lacking. To measure adhesion, we present here a protocol that is robust to variations in protocol parameters, pupal age and calculation methods. We find that the glue, which covers the entire pupal surface, increases the animal rigidity and plasticity when bound to a glass slide. Our survey of pupal adhesion in 25 Drosophilidae species reveals a wide range of phenotypes, from species that produce no or little glue and adhere little, to species that produce high amounts of glue and adhere strongly. One species, D. hydei, stands out from the rest and emerges as a promising model for the development of future bioadhes..., , , # Dataset for \"Adhesive and mechanical properties of the glue produced by 25 Drosophila species\" [https://doi.org/10.5061/dryad.79cnp5j3j](https://doi.org/10.5061/dryad.79cnp5j3j) This dataset comprises all the supporting files associated with the manuscript \"Adhesive and mechanical properties of the glue produced by 25 Drosophila species\" by Monier et al. ## Description of the data and file structure ### File S1. FileS1\_dorsal\_views\_light\_pupae.zip (1.99 GB).Dorsal views of light pupae. Compressed zip file of 1178 pupae pictures from the dorsal view (.tiff and .jpg). This file contains pictures of pupae manually selected for their light pupal case colour. The name of each image corresponds to the single pupa id, which is the date of adhesion test in format YYYYMMDD followed by a two-digit number which corresponds to the order in which pupa were processed on a given day. To find the corresponding species and associated information, please check Table “metadata_concatenate.csv” ...果蝇胶（Drosophila glue）是蝇幼虫为附着于基质长达数日而分泌的生物粘合剂，近来因其独特的粘附与力学特性受到广泛关注。尽管果蝇胶的分泌现象早在50余年前就已被报道，但目前仍缺乏针对不同果蝇物种来源的这类蛋白凝胶的粘附与力学特性的系统性调研。 为量化粘附性能，本文提出了一种对实验参数、蛹龄及计算方法均具有鲁棒性的实验方案。研究发现，覆盖蛹体全表面的果蝇胶在附着于玻璃载玻片后，可提升蛹体的刚度与可塑性。 本研究对25种果蝇科（Drosophilidae）物种的蛹粘附性能开展调研，结果显示其表型跨度极大：从几乎不分泌胶、粘附力极弱的物种，到大量合成胶蛋白、粘附能力极强的物种均有分布。其中海德果蝇（D. hydei）表现尤为突出，有望成为未来生物粘合剂研发的理想模型…… 本数据集为《25种果蝇所分泌胶的粘附与力学特性》一文的配套数据集，相关DOI链接为：https://doi.org/10.5061/dryad.79cnp5j3j。 本数据集包含Monier等人发表的题为《25种果蝇所分泌胶的粘附与力学特性》的研究论文的全部配套支持文件。 ## 数据与文件结构说明 ### 补充文件S1 FileS1_dorsal_views_light_pupae.zip（1.99 GB）：该压缩包收录了浅色蛹的背视图图像，内含1178张蛹的背视图照片，格式为.tiff与.jpg。所收录的蛹均为人工筛选出的蛹壳颜色较浅的个体。每张图片的文件名对应单个蛹的唯一ID：其前缀为粘附测试日期（格式为YYYYMMDD），后缀为两位数字，代表当日实验中该蛹的处理顺序。如需查询对应物种及相关信息，请查阅"metadata_concatenate.csv"表格。