Experimental dataset: "Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure"
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This repository contains experimental data supporting our paper "Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure". Original Publication:Tah, I., Haertter, D., et al. (2024). Minimal vertex model explains how the amnioserosa avoids fluidization during Drosophila dorsal closure. PNAS (in press).Preprint available at: https://doi.org/10.1101/2023.12.20.572544 Dataset Structure amnioserosa_timelapses/ This directory contains confocal microscopy time-lapse recordings of eCadherin-labeled Drosophila melanogaster embryos during dorsal closure: Imaging parameters: Frame interval: 15 seconds Pixel size: 0.158 µm Data files: *_data.p: Morphometric features of individual amnioserosa cells during closure *_junctions.p: Morphometric features of individual cell-cell adherens junctions read_filter_data.py: Python script demonstrating data reading and filtering procedures junction_laser_cuts/ This directory contains high-speed recordings of UV-laser junction ablation experiments: Imaging parameters: Frame rate: 5 Hz Pixel size: 0.364 µm Content: Time series of eCadherin-labeled embryos before, during, and after precise UV-laser severing of individual junctions For detailed experimental methods and protocols, please refer to the published paper.
