Data for: Anti-Inflammatory Effects of Interleukin-4 on Intervertebral Disc Cells

Sheet 1 corresponds to the data for Figure 2: measuring the phosphorylation of STAT6 using ELISA. Sheet 2 corresponds to the data for Figure 3: measuring IL-4 induced gene expression using Real-time RT-PCR. Sheet 3 corresponds to the data for Figure 4: measuring IL-4 inhibition of LPS stimulated gene expression using Real-time RT-PCR. Sheet 4 corresponds to the data for Figure 5: measuring IL-4 inhibition of LPS stimulated IL-6 and IL-8 protein release in the conditioned media using ELISA.工作表1对应图2的实验数据：采用酶联免疫吸附试验（ELISA）检测信号转导与转录激活因子6（STAT6）的磷酸化水平。工作表2对应图3的实验数据：采用实时逆转录聚合酶链式反应（Real-time RT-PCR）检测白细胞介素4（IL-4）诱导的基因表达。工作表3对应图4的实验数据：采用实时逆转录聚合酶链式反应（Real-time RT-PCR）检测白细胞介素4对脂多糖（LPS）刺激诱导的基因表达的抑制作用。工作表4对应图5的实验数据：采用酶联免疫吸附试验（ELISA）检测白细胞介素4对脂多糖刺激的细胞培养上清液中白细胞介素6（IL-6）与白细胞介素8（IL-8）蛋白释放的抑制作用。