Characterization of a diffuse low and high grade glioma cell line biobank

Diffuse low and high grade glioma are primary brain tumors arising from gllial cells. They are subdivided into three groups: IDH1-mutant oligodendrogliomas, IDH1-mutant astrocytomas and IDH1-Wild Type glioblastomas. Cellular tools to study these tumors remains poors, specially for low grades gliomas. We constituted a biobank of 12 patient-derived cell lines, from IDH1-mutant and wild type gliomas, then charactrized them to test their relevance as in vitro models. Overall design: To characterized the transcriptomic profile of each cell line, we performed an RNA sequencing after culture the cell in presence or absence of growth factors EGF and FGF2