Large-scale, high-efficiency production of coffee somatic embryos

Abstract The study aims to compare the efficiency of previously used liquid media for coffee, evaluating NAA concentrations in liquid medium and proline in semi-solid medium in the regeneration of somatic embryos and asses concentrations of BA and IAA in the maturation of embryos in temporary immersion bioreactors. For the regeneration of globular embryos from embryogenic aggregates and calli, we tested five concentrations (0.00, 0.25, 0.50, 1.00 and 2.00 mg L-1) of NAA in liquid medium and five concentrations (0.0, 0.5, 1.0, 2.0 and 4.0 g L-1) of proline in semisolid medium. The multiplication of embryogenic aggregates was highest in culture medium MM, reaching a density 7.5 times greater than that of the initial density. NAA promoted a linear increase in embryo regeneration. The medium containing 2.0 mg L-1 BA and 0.0 mg L-1 IAA yielded the highest percentage of large cotyledonary embryos.摘要 本研究旨在对比既往用于咖啡培养的液体培养基的应用效率，评估体细胞胚(somatic embryos)再生过程中液体培养基内萘乙酸(NAA)的浓度与半固体培养基内脯氨酸的作用，并检测临时浸没生物反应器中胚成熟阶段苄基腺嘌呤(BA)与吲哚乙酸(IAA)的浓度。针对从胚性聚集体与愈伤组织再生球形胚的实验，我们分别设置了5组液体培养基萘乙酸浓度梯度（0.00、0.25、0.50、1.00及2.00 mg·L⁻¹），以及5组半固体培养基脯氨酸浓度梯度（0.0、0.5、1.0、2.0及4.0 g·L⁻¹）。胚性聚集体在MM培养基中的增殖效果最佳，其最终密度可达初始密度的7.5倍。萘乙酸可使胚再生率呈线性提升。当培养基中添加2.0 mg·L⁻¹苄基腺嘌呤与0.0 mg·L⁻¹吲哚乙酸时，大型子叶胚的诱导率达到最高。