Holstein cattle rumen whole metagenome sequence Madison, WI

A comparison of the efficacy of long-reads and proximity ligation in resolving members of the cattle rumen microbial community. A combined rumen fluid and solid sample was sequenced using short- and long-read DNA sequencing technologies and each dataset was assembled de novo, individually. Additionally, proximity ligation was used as a means of identifying microbial genome structure and novel biological features, such as viral-host specificity in the sample.

本文对长读长测序技术与邻近连接技术解析牛瘤胃微生物群落成员的有效性进行了比较研究。通过对瘤胃流体和固体样本采用短读长与长读长DNA测序技术进行测序，并对每个数据集进行独立的新组装。此外，邻近连接技术被用作识别微生物基因组结构以及样本中的新型生物特征的手段，例如病毒宿主特异性。