Estimating the distribution of fitness effects of loss of heterozygosity (LOH) events using an engineered library of Saccharomyces cerevisiae

Loss of heterozygosity (LOH) is an important source of genetic variation in asexual diploid organisms, yet the full spectrum of its fitness effects remains largely uncharacterized. To systematically investigate the distribution of fitness effects (DFE) of LOH, we engineered a diverse, barcoded library of diploid Saccharomyces cerevisiae strains, each containing randomly induced LOH events.