The effect of anti-σ70 factors on mucoid induction in strains of P. aeruginosa.

M and NM represent a mucoid and a non-mucoid phenotype, respectively, after incubation on PIA plates supplemented with 300 µg/ml of carbenicillin and 0.1% L-Ara at 37°C for 24 hrs. The quantity of alginate production was measured (mean ± standard error, µg/ml/OD600) and listed in the brackets.athe frameshift mutation in mucA results in the fusion of a truncated MucA (125 amino acids of N-terminal MucA) with an additional 3 amino acids with no homology to the amino acid sequence of wild type MucA.ba concentration of 0.5% L-Ara was needed to induce the conversion to mucoidy in CF149. The alginate production of all AlgQ-overexpressing strains was measured on PIA plates containing 300 µg/ml of carbenicillin and 0.5% L-Ara.