RNA sequencing of nuclease dead PET127 conditions

PET127, is thought to be a mitochondrial RNA exonuclease and loss of PET127 is thought to cause accumulation of untrimmed RNA in the mitochondria. Here we show RNA sequencing of Saccharomyces cerevisiae in four PET127 conditions: PET127 wild-type, pet127∆, pet127-nd (nuclease dead)/pet127∆, and pet127-nd/PET127. The resulting sequences show that regions of mitochondrial RNA accumulate in the pet127∆ background, and the RNA sequencing profile of PET127 containing disruptive mutations in the conserved exonuclease active site mimics that of pet127∆.