Gene expression profiles of basophil progenitors, CD34- pre-basophils and mature basophils

In this study, we explored gene expression profiles of CD34-CLEC12Ahi pre-basophils isolated from the bone marrow as well as CD34-CLEC12Alo mature basophils from the bone marrow and CD34+ basophil progenitors. We revealed that the gene expression of mature basophils isolated from the bone marrow and spleen resembled each other whereas pre-basophils and mature basophils showed distinct gene expression profiles. Overall design: We isolated CD34-CLEC12AhiCD9lo pre-basophils from the bone marrow as well as CD34-CLEC12AloCD9hi mature basophils from the bone marrow and spleen separately and compared their gene expression profiles using bulk RNA-seq analysis. As reference, we also included sort-purified CD200R3+cKit-CLEC12AhiCD34+ bone marrow cells that correspond to uni-potent basophil progenitors (BaPs).